Seed Train Optimization for Cell Culture
B. Frahm, in: R. Pörtner (Ed.), Animal Cell Biotechnology - Methods and Protocols, 3rd ed., Humana Press, Totowa, NJ, 2013, pp. 355–367.
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Pörtner, Ralf
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Abstract
For the production of biopharmaceuticals a seed train is required to generate an adequate number of cells for inoculation of the production bioreactor. This seed train is time- and cost-intensive but offers potential for optimization. A method and a protocol are described for the seed train mapping, directed modeling without major effort, and its optimization regarding selected optimization criteria such as optimal points in time for cell passaging. Furthermore, the method can also be applied for the set-up of a new seed train, for example for a new cell line. Although the chapter is directed towards suspension cell lines, the method is also generally applicable, e.g. for adherent cell lines.
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Animal Cell Biotechnology - Methods and Protocols
Band
1104
Seite
355–367
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Frahm B. Seed Train Optimization for Cell Culture. In: Pörtner R, ed. Animal Cell Biotechnology - Methods and Protocols. Vol 1104. 3rd ed. Methods in Molecular Biology. Humana Press; 2013:355-367. doi:10.1007/978-1-62703-733-4_22
Frahm, B. (2013). Seed Train Optimization for Cell Culture. In R. Pörtner (Ed.), Animal Cell Biotechnology - Methods and Protocols (3rd ed., Vol. 1104, pp. 355–367). Humana Press. https://doi.org/10.1007/978-1-62703-733-4_22
Frahm B (2013) Seed Train Optimization for Cell Culture. In Pörtner R (ed.), Animal Cell Biotechnology - Methods and Protocols, 3rd ed., vol. 1104. Totowa, NJ: Humana Press, pp. 355–367.
Frahm, Björn. “Seed Train Optimization for Cell Culture.” In Animal Cell Biotechnology - Methods and Protocols, edited by Ralf Pörtner, 3rd ed., 1104:355–67. Methods in Molecular Biology. Totowa, NJ: Humana Press, 2013. https://doi.org/10.1007/978-1-62703-733-4_22.
Frahm, Björn. 2013. Seed Train Optimization for Cell Culture. In: Animal Cell Biotechnology - Methods and Protocols, hg. von Ralf Pörtner, 1104:355–367. 3. Aufl. Methods in Molecular Biology. Totowa, NJ: Humana Press. doi:10.1007/978-1-62703-733-4_22, .
Frahm, Björn: Seed Train Optimization for Cell Culture. In: Pörtner, R. (Hrsg.): Animal Cell Biotechnology - Methods and Protocols, Methods in Molecular Biology. Bd. 1104. 3. Aufl. Totowa, NJ : Humana Press, 2013, S. 355–367
B. Frahm, Seed Train Optimization for Cell Culture, in: R. Pörtner (Ed.), Animal Cell Biotechnology - Methods and Protocols, 3rd ed., Humana Press, Totowa, NJ, 2013: pp. 355–367.
B. Frahm, “Seed Train Optimization for Cell Culture,” in Animal Cell Biotechnology - Methods and Protocols, 3rd ed., vol. 1104, R. Pörtner, Ed. Totowa, NJ: Humana Press, 2013, pp. 355–367. doi: 10.1007/978-1-62703-733-4_22.
Frahm, Björn. “Seed Train Optimization for Cell Culture.” Animal Cell Biotechnology - Methods and Protocols, edited by Ralf Pörtner, 3rd ed., vol. 1104, Humana Press, 2013, pp. 355–67, https://doi.org/10.1007/978-1-62703-733-4_22.
Frahm, Björn: Seed Train Optimization for Cell Culture, in: Pörtner, Ralf (Hg.): Animal Cell Biotechnology - Methods and Protocols, Bd. 1104, Totowa, NJ 32013 (Methods in Molecular Biology), S. 355–367.
Frahm B. Seed Train Optimization for Cell Culture. In: Pörtner R, editor. Animal Cell Biotechnology - Methods and Protocols. 3rd ed. Totowa, NJ: Humana Press; 2013. p. 355–67. (Methods in Molecular Biology; vol. 1104).
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